"We are ready for dialogue; we accept an equal conversation that considers our dignity and wisdom, and we will not submit to force in any way," Masoud Pezeshkian told NBC in an interview in Tehran.
His official website framed the remarks as referring to the United States and European Union.
"The Islamic Republic of Iran is committed to peace and de-escalation in the region and globally, condemns the Zionist regime's war-mongering, aggression, and genocide, and stands ready for honorable and equal negotiations," he added.
The interview was broadcast on the American television network on Wednesday in spite of the fact the two nations have no diplomatic ties and Iran's Supreme Leader has spoken out against negotiating with the US.
Pezeshkian has consistently advocated for regional and global peace, as the nation's armed allies across the region face increasing challenges.
“We desire with the countries of the region and the world, and we are striving to establish peace and security both inside and outside the country,” he said last month during a visit to North Khorasan province.